Jinma Xinbao: Antigen capture method to help you choose Elisa kit - Database & Sql Blog Articles

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At present, there are many types of elisa kits on the market, which are popular among scientific research workers because of their special repeatability, strong heterogeneity and high sensitivity. There are countless varieties of elisa kits available on the market. Let everyone have a selective syndrome, do not know which ELISA kit is easy to use, do not know how to judge the quality of a kit, and ultimately can not pick the most suitable kit. In view of the above situation, our company lists some of the common knowledge of Elisa kits, hope to provide a reference for everyone to buy.

Although there are many types of ELISA kits, they all have one thing in common, that is, antigen capture. Typical capture formats include adsorption of the antigen to a microplate or capture with antibodies on a microplate. In-cell ELISA and enzyme-linked immunospot ELISPOT are two special types. In-cell ELISA requires immobilization and permeabilization of cells cultured in microplates, followed by in situ detection with antibodies. ELISPOT is a bit like Western blotting. Cells are first cultured in membrane-coated microplates, and then the antigen secreted by the cells is detected on the membrane to form spots. In addition, we also need to select 96-well plates or 384-well plates for ELISA experiments according to their needs. Both monoclonal and polyclonal antibodies can be used in ELISA kits, and in fact sometimes the combination is better. For example, for the double-anti-sandwich method, it is sometimes more helpful to use a polyclonal antibody for capture and a monoclonal antibody for detection. Polyclonal antibodies ensure that all antigens are captured, and then the monoclonal antibodies specifically detect antigens that possess specific epitopes. Since its establishment, Shanghai Jinma has been providing sincere service to every customer with its honest and professional business philosophy. Our service philosophy is: Do not leave the word of integrity, Henggu so far, only integrity can win the final victory! Thank you for your great support to the company over the years! I hope that through our unremitting efforts, we can make greater contributions to the cause of scientific research!