Raise the attention of cells (1) - Huaqiang Electronic Network

(1) How should the freezing tube be thawed?

Immediately after taking out the cryotube, it should be quickly thawed in a 37 °C water tank. Gently shake the cryotube to melt it in 1 minute. Note that the water surface should not exceed the edge of the cryotube cover, otherwise it will be prone to contamination. When the chilled tube is removed from the liquid nitrogen drum, it must be safe to prevent the freezing of the freezing tube.

(2) How to treat DMSO when the cell cryotube is thawed

After the cells are thawed, the DMSO can be directly removed by centrifugation, suspended in a fresh medium, and directly placed in a culture flask containing fresh medium for cell culture, thereby preventing cell growth from being affected by DMSO after thawing.

(3) Whether it is possible to use a medium different from the original culture conditions

Each cell strain has its own specific and adapted cell culture medium. If it is used suddenly and the culture medium originally provided with different culture conditions, the cells are mostly unable to adapt immediately, causing the cells to be unable to survive and should not be replaced immediately.

(4) Whether serum types different from the original culture conditions can be used

Serum is an extremely important source of nutrients in cell culture, so the type and quality of serum can have a significant impact on cell growth. Serum from different species varies in the amount or content of some substances or molecules, and errors in serum use often cause cells to fail to survive.

(5) What is FBS, FCS, CS, HS?

FBS (fetal bovine serum) and FCS (fetal calf serum) have the same meaning, both refer to fetal bovine serum. CS (calf serum) refers to calf serum. HS (horseserum) refers to horse serum.

(6) 5% or 10% CO should be used when culturing cells

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Most of the general medium uses HCO ₃ ^- /CO ₃ ^2- /H ⁺ as the pH buffer system, while NaHCO in the medium

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The content will determine the CO that should be used in cell culture.

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concentration. Theory when the medium is NaHCO

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When the content is 3.7 g per liter, 10% CO should be used for cell culture.

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When the medium is NaHCO

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For 1.5 g per liter, 5% CO should be used

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Cultured cells.